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来源 用途 交叉反应性 分子量 Rabbit WB, IP, IF, IHC, ICC H, M, R 70.0kDa        WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.        H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.        配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。        建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)： WB IP IF IHC ICC FC ELISA ChIP 1:1,000 1:10-1:50 1:50-1:200 1:50-1:500 1:50-1:200 - - - 抗体详细信息如下: About this Antibody Name Lamin B1 Rabbit Monoclonal Antibody Category Rabbit Monoclonal Antibody (RabMAb); Primary antibody Isotype IgG Purification Affinity purification About the Immunogen Immunogen Recombinant protein Gene ID 4001(Human); 16906(Mouse); 116685(Rat) SwissProt P20700(Human); P14733(Mouse); P70615(Rat) Synonyms ADLD; LMN; LMN2; LMNB Category Apoptosis Background A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, termed Ced-3/ICE, functions as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Nuclear lamins are critical to maintaining the integrity of the nuclear envelope and cellular morphology as components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family. 包装清单: 产品编号 产品名称 包装 AF1408 Lamin B1 Rabbit Monoclonal Antibody 50μl AZ050 Western一抗稀释液 50ml - 说明书 1份 保存条件:        Lamin B1 Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。 注意事项:        如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。        回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。        提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得的检测效果，请考虑使用上述检测专用的一抗稀释液。        本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。        为了您的安全和健康，请穿实验服并戴一次性手套操作。